Human Glutathione Peroxidase (GSH-PX) ELISA Kit

Samples serum, plasma, tissue homogenates, cell culture supernates Principle of test The kit is for the quantitative level of GSH-PX in the sample, adopt purified Human GSH-PX to coat microtiter plate, make solid-phase antibody, then add GSH-PX to wells, Combine GSH-PX antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of GSH-PX in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components Microelisa Stripplate(12well×8strips) Standard (1×0.5ml) Standard Diluent(1×1.5ml) HRP-Conjugate Reagent(1×6ml) Sample Diluent(1×6ml) Chromogen Solution A(1×6ml) Chromogen Solution B(1×6ml) Stop Solution(1×6ml) Wash Solution(1×20ml×30 fold) User manual(1) Adhesive Strip(2) USAGE For Research Use Only! TAG: Glutathione Peroxidase ShareTo： Facebook Twitter Google+ Previous　Human Urea Transporter 2 (SLC14A2) ELISA Kit　 Next　Human Glutathione S Transferase alpha (GSTa) ELISA Kit