PRLR-JAK1 Functional Assay Kit

CTK Functional cell lines are engineered to co-a ProLink (PK) tagged Cytokine receptor, an untagged CTK, and an Enzyme Acceptor (EA) tagged SH2 domain. Activation of the Receptor-PK induces phosphorylation of the receptor by the CTK. This leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance. https://www.creativebiomart.net/prlr-jak1-functional-assay-kit-463607.htm